Reporter
Ribo-TetA

Part:BBa_J119140:Design

Designed by: Sachith Dharmasena and Todd Eckdahl   Group: Eckdahl Lab   (2013-02-08)


Theophylline Riboswitch - tetracycline resistance protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 306
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 452
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 478
    Illegal NgoMIV site found at 846
    Illegal NgoMIV site found at 1006
    Illegal AgeI site found at 100
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BsaI sites were introduced into PCR products, but are not present in the final construct.


Source

Constructed using BsaI GGA of PCR products from J100065 and J31007.

References